written by who? HindIII is a form of e coli and it was in the verification code. wow am I on a limb? I feel like the tree is being sawed down. "whatever". I get my entertainment by refreshing the White House contact verification code and trying to decipher it. It can be strange but I find it fun.the UN was meantioned and who to pay . a maid or deral? interesting. we are again being screwed(again) and the herded ducks quack frantically but do nothing to stop it.
Here is some wiki info on Hind III they also had dates and numbers. 55 repeated itself. I think they were referring to dates and coordinates 'date' was in one of the codes.
Crystallographic structure of the HindIII restriction endonuclease dimer (cyan and green) complexed with double helical DNA (brown) based on the PDB 2E52 coordinates.
Identifiers
Symbol hindIIIR
Entrez 950303
PDB 2e52
UniProt P43870
Other data
EC number 3.1.21.4
HindIII is a type II site-specific deoxyribonuclease restriction enzyme isolated from Haemophilus influenzae that cleaves the palindromic DNA sequence AAGCTT in the presence of the cofactor Mg2+ via hydrolysis.[1]
The cleavage of this sequence between the AA's results in 5' overhangs on the DNA called sticky ends:
5'-A |A G C T T-3'
3'-T T C G A| A-5'
Restriction endonucleases are used as defense mechanisms in prokaryotic organisms in the restriction modification system. Their primary function is to protect the host genome against invasion by foreign DNA, primarily bacteriophage DNA. There is also evidence that suggests the restriction enzymes may act alongside modification enzymes as selfish elements, or may be involved in genetic recombination and transposition.[2]
Contents
[hide]
* 1 Enzyme Structure
* 2 Site-directed mutagenesis
* 3 Proposed mechanism
* 4 Uses in research
